| Birmingham Legion FC vs. Louisville City FC | 3-15-2025
Saturday
TBD | Protective Stadium in Birmingham, AL | View Tickets |
| Louisville City FC vs. Loudoun United FC | 3-22-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Detroit City FC | 3-29-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Sacramento Republic FC vs. Louisville City FC | 4-5-2025
Saturday
TBD | Heart Health Park in Sacramento, CA | View Tickets |
| Louisville City FC vs. Richmond Kickers SC | 4-26-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Lexington Sporting Club | 5-1-2025
Thursday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Pittsburgh Riverhounds SC | 5-10-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Monterey Bay FC vs. Louisville City FC | 5-24-2025
Saturday
TBD | Cardinale Stadium in Marina, CA | View Tickets |
| Loudoun United FC vs. Louisville City FC | 5-31-2025
Saturday
TBD | Segra Field in Leesburg, VA | View Tickets |
| Louisville City FC vs. Tampa Bay Rowdies | 6-7-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Rhode Island FC | 6-11-2025
Wednesday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Birmingham Legion FC | 6-14-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Loudoun United FC vs. Louisville City FC | 6-25-2025
Wednesday
TBD | Segra Field in Leesburg, VA | View Tickets |
| Louisville City FC vs. North Carolina FC | 6-28-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. FC Tulsa | 7-19-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Lexington Sporting Club vs. Louisville City FC | 7-26-2025
Saturday
TBD | Lexington SC Stadium in Lexington, KY | View Tickets |
| Louisville City FC vs. North Carolina FC | 8-2-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Charleston Battery | 8-9-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Miami FC vs. Louisville City FC | 8-16-2025
Saturday
TBD | Pitbull Stadium in Miami, FL | View Tickets |
| Louisville City FC vs. New Mexico United | 8-23-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Indy Eleven vs. Louisville City FC | 8-30-2025
Saturday
TBD | Carroll Stadium in Indianapolis, IN | View Tickets |
| Rhode Island FC vs. Louisville City FC | 9-6-2025
Saturday
TBD | Tidewater Landing Stadium in Pawtucket, RI | View Tickets |
| Louisville City FC vs. Las Vegas Lights FC | 9-20-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Indy Eleven | 10-4-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Miami FC | 10-11-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Hartford Athletic FC | 10-25-2025
Saturday
TBD | Lynn Family Stadium in Louisville, KY | View Tickets |
